Journal: Biochemistry

Article Title: Allosteric Inhibition of the Epidermal Growth Factor Receptor

doi: 10.1021/acs.biochem.0c00978

Figure Lengend Snippet: EGFR645–1186 is a model for the interaction of E1S with full length EGFR in cells. (A) Silver Stain blots illustrating the relative concentration of EGFR fragments EGFR645–1186, EGFR672–1186, or EGFR1–621 (0.15 μM) incubated with 0 or 100 equivalents DBE1S or BDE1S for 2 h, then irradiated (365 nm,10 min). Samples were resolved by SDS-PAGE (10% polyacrylamide) and stained with Silver Stain PlusTM (BioRad) (B) Immunoblots illustrating the relative amounts of biotinylated fragments EGFR645–1186, EGFR672–1186, or EGFR1–621 post- incubation with 0, 1, 10, 50 or 100 equivalents of DBE1S or BDE1S for 2 h, irradiated, resolved and immunoblotted using an anti-Biotin antibody. See also Figure S3.

Article Snippet: Samples were resolved by SDS-PAGE (10% polyacrylamide) and stained with Silver Stain PlusTM (BioRad) (B) Immunoblots illustrating the relative amounts of biotinylated fragments EGFR645–1186, EGFR672–1186, or EGFR1–621 post- incubation with 0, 1, 10, 50 or 100 equivalents of DB E1 S or BD E1 S for 2 h, irradiated, resolved and immunoblotted using an anti-Biotin antibody.

Techniques: Silver Staining, Concentration Assay, Incubation, Irradiation, SDS Page, Staining, Western Blot

Journal: European journal of biochemistry

Article Title: The relevance of N-linked glycosylation to the binding of a ligand to guanylate cyclase C.

doi: 10.1046/j.1432-1327.1999.00488.x

Figure Lengend Snippet: Fig. 2. SDS/PAGE and Western blotting of ECD6H expressed from Sf21 cells. Purified by concanavalin A and Ni-chelating affinity chromatography, and visualized by (A) silver stain, (B) a His6-tagged protein detection kit and (C) concanavalin A-conjugated HRP. PGNase F (+) and (±) indicate treatment without or with PNGase F. (D) ECD6H was treated without (lanes 1, 2) or with (lanes 3, 4) PNGase F and subsequently photoaffinity-labeled with BB-[125I-Tyr4]STp(4±17) in the absence (lanes 1, 3) and presence (lanes 2, 4) of 1026 m STp(4±17).

Article Snippet: The deglycosylation of ECD6H was confirmed using succinylatedconcanavalin A conjugated with horseradish peroxidase (HRP; Vector Lab. Inc.) and an HRP Conjugate Substrate Kit (BioRad).

Techniques: SDS Page, Western Blot, Purification, Affinity Chromatography, Silver Staining, Labeling

Journal: bioRxiv

Article Title: Tyrosine phosphorylation coupling of one carbon metabolism and virulence in an endogenous pathogen

doi: 10.1101/2025.03.11.642667

Figure Lengend Snippet: a, b) Coomassie blue stain of representative SDS-PAGE of P. gingivalis cell fractions analyzed in . c) Silver stain of representative SDS-PAGE of P. gingivalis culture supernatants. The intense band is the catalytic domain from the gingipains.

Article Snippet: Proteins were visualized with Coomassie Brilliant Blue (BioRad) or silver stain (ThermoFisher).

Techniques: Staining, SDS Page, Silver Staining